TLC-MS Interface 2, CAMAG

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TLC-MS Interface 2

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Full description

Through the pioneering concept of hyphenating High-Performance Thin-Layer Chromatography with Mass Spectrometry unequivocal substance identification is possible. The CAMAG TLC-MS Interface 2 can be installed plug & play with any LC-MS system without adjustments or mass spectrometer modifications. Depending on the MS system, a substance can be identified within a minute via its mass spectrum, or for an unknown substance zone, the respective sum formula can be obtained. Furthermore, interesting zones can be eluted into vials for further investigations with, e.g., NMR, (ATR-)FTIR, ESI-MS, and MALDI-MS.

The chromatogram zones are eluted from the HPTLC plate with methanol or another suitable solvent with the flow speed appropriate for the LC-MS system. The round elution head is used for circular zones and the oval elution head for zones in the form of bands. After elution the eluate is either directly transferred to the mass spectrometer or collected in a sample vial for further offline analysis.

The CAMAG TLC-MS Interface 2 features a modified elution head and an easily accessible, exchangeable filter, arranged in front of the valve. Cleaning is facilitated as compared to the previous version, making it highly efficient. By pushing a button, the elution path is cleaned of matrix particles with compressed air, increasing the lifetime of the filter and preventing the system from becoming blocked. These filters can be easily replaced without any modification to the elution head.

Plate positioning is significantly simplified: together with the positioning scale and the adjustable plate-stopper, the integrated crosshair laser enables accurate and reproducible positioning of the plate. Alternatively the coordinates determined by the CAMAG TLC Visualizer 2 or the CAMAG TLC Scanner 4 can be used to precisely position the HPTLC plate.

Key features
• Rapid and contamination-free elution of selected zones
• Direct transfer to the mass spectrometer
• Plug & play installation
• Compatible with any LC-MS system
• Confirmation of known substances within a minute
• Highly effective backwashing function prevents the elution path from becoming blocked
• Easy handling ensures accurate and reproducible plate positioning
• Low solvent consumption

Example
Characterization of separated compounds by Mass Spectrometry
(Steviol glycosides in Stevia formulations*)
A: Chromatogram for localizing the zones (derivatized with ß-naphtol reagent)
B: HPTLC plate after elution of zones with the TLC-MS Interface 2
C: HPTLC-ESI-MS spectra of Rebaudioside A, m/z 989.6 [M ⁺ Na]⁺;

Product specification

Model	TLC-MS Interface 2
Dimensions and weight with	W x D x H: 275 x 425 x 275 mm, 14.5 kg
standard plate support
Laser crosshairs	Laser: 5 mW, class 2M, battery operated (two batteries 1.5 V,
	AA or LR6), operating time on batteries: up to 100 hours
Materials	Elution head: made of passivated stainless steel,
	resistant to all common solvents
Requirements	Gas connection: compressed air or Nitrogen 4–6 bar
	Solvent flow rate: 50–300 μL/min
	Pressure of elution head onto HPTLC plate: max 400 N