Litesizer™, ANTON PAAR

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Litesizer™

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The Litesizer series: Two instruments, up to five modes

The Litesizer™ particle analyzers open up many particle analysis options: You can use the instruments in up to five different modes to measure particle size, zeta potential, molecular mass, transmittance, and refractive index.
Particle analysis at the touch of a button.

The Litesizer™ is an instrument for characterizing nano- and microparticles in dispersions and solutions. Litesizer™ 100 determines particle size by measuring dynamic light scattering (DLS). Litesizer™ 500 determines particle size, zeta potential, and molecular mass by measuring dynamic light scattering (DLS), electrophoretic light scattering (ELS), and static light scattering (SLS). Moreover, the Litesizer™ 500 is the only DLS-based particle analyzer that is able to perform a straightforward measurement of the sample’s refractive index.

A highlight of the Litesizer™ is its ingeniously simple software. We have created a one-page workflow, where input parameters, results and analysis are all on a single page: You can set up your experiments in a few seconds, and produce the analyses and reports you need at the touch of a button.

Litesizer Key Features

Particle-size measurements: Unprecedented resolution
Several different particle sizes in a single suspension can be precisely resolved, thanks to highly developed algorithms in the firmware.

Continuous transmittance measured automatically
Transmittance gives you instant feedback about your sample, and allows automatic optimization of measurement parameters (angle, focus position, and measurement duration).

Ingeniously simple software, Kalliope™
Kalliope™ provides an intuitive one-page workflow, where input parameters, measurements and analyses are all at hand. In addition, Kalliope™ is fully compliant with the US FDA's 21 CFR part 11 regulations.

Litesizer™ 500 special Key Features

Accurate determination of the refractive index
While for most particle analyzers these indices must be determined from external sources, the Litesizer™ 500 is able to measure the refractive index for the exact wavelength and temperature of your experiment. This ensures maximum accuracy of particle size and zeta potential results under all experimental conditions.
The Litesizer™ 500 determines the solvent refractive index within ±0.5 %, as defined by ISO 22412:2017 concerning the accuracy of the refractive index required for DLS.

cmPALS – new patented technology for zeta potential
With cmPALS (European Patent 2 735 870), measurements can be performed at much lower applied electric fields, and within significantly shorter measuring times, thereby enabling reliable measurements of even highly sensitive samples.

Unique Ω-shaped capillary tube for zeta potential
Measurements are more stable and reproducible because of the unique Ω-shaped capillary in the cuvette.

Optional accessory for Litesizer 500

Dosing System

The Dosing System is an optional accessory for the Litesizer™ 500 that automates the adjustment of the sample’s pH directly in the measurement cuvette. Fast and accurate characterization of zeta potential and particle size changes in response to pH are now possible. The Dosing System is used together with the flow module.

Automating the pH adjustment process not only saves time and effort but also considerably reduces the possibility of human error.

High accuracy and reproducibility
• The pH is modified automatically, reducing human error.
Fully programmable
• The Dosing System is steered by the dedicated software Kalliope™. The integrated templates for pH ramps help you set up your experiment quickly and precisely.
Traceable
• Reports and graphs are automatically generated.
Easy maintenance
• The self-cleaning function ensures that no carryover of sample from the previous measurement can occur.

Product specification

Model	Litesizer™100	Litesizer™ 500
General specifications
Temp. control range	0 – 90 °C	0 – 90 °C
Light source	Laser / 40 mW, 658 nm	Laser / 40 mW, 658 nm
Laser warm-up time	6 min	6 min
Ambient operating temp.	10 – 35 °C	10 – 35 °C
Humidity, non-condensing	up to 90 %	up to 90 %
Dimensions (W, D, H)	460 mm, 485 mm, 135 mm	460 mm, 485 mm, 135 mm
Weight	approx. 16 kg	approx. 18 kg
Particle size specifications
Measuring range (particle diameter)	0.3 nm – 10 μm*	0.3 nm – 10 μm*
Sensitivity
min. conc. (protein, lysozyme)	0.1 mg/mL	0.1 mg/mL
min. conc. (polymer, polystyrene latex)	1 µg/mL	1 µg/mL
Maximum sample concentration	40 % w/v	indicated below
Accuracy, relatively to certified reference material	Better than +/-2 %	Better than +/-2 %
Repeatability, relatively to certified reference material	Better than +/-2 %	Better than +/-2 %
Minimum sample volume	12 µL	12 µL
Measurement angles	175°	15°, 90°, 175°
Zeta potential specifications
Measuring range	-	-600 to +600 mV
Mobility	-	10^-11 m²/V.s to 2 x 10^-7 m²/V.s
Size range	-	3.8 nm – 100 µm
Sensitivity	-	1 mg/mL (lysozyme)
Maximum sample concentration	-	40 % w/v
Sample volume	-	350 µL
Maximum sample conductivity	-	200 mS/cm
Molecular mass specifications
Measuring range (mass)	-	980 Da – 20 MDa
Measuring range (particle size)	-	up to 40 nm (diameter)
Sensitivity	-	0.1 mg/mL (lysozyme)
Accuracy	-	+/-10 %
Repeatability	-	+/-5 %
Measurement angle	-	90°
Transmittance specifications
Size range	No limit	No limit
Measuring time	10 s	10 s
Minimum sample volume	15 µL	15 µL
Refractive index specifications
Measuring range	-	1.28 to 1.50
Accuracy	-	+/-0.5 %
Temperature range	-	0 °C to 90 °C
Wavelength	-	658 nm
Minimum sample volume	-	1 mL
Wetted parts:	-	Quartz, glass

*under laboratory conditions

Optional accessory	Dosing System
Environmental conditions
Sample temperature range	10 °C to 50 °C
Ambient temperature range	10 °C to 35 °C
Relative humidity	35 % to 80˙% non-condensing
Sample compatibility	Aqueous
pH conditions
Standard pH electrode type	Flat membrane
pH range	1 to 13 (sample-dependent)
Dosing specifications
Number of supported additives	2
Standard sample beaker volume	Max. 150 mL
Standard additive bottle volume	Max. 1000 mL
Minimum sample volume	5 mL
Syringe volume	5 mL
Minimum additive volume	2.5 µL
Dosing drive resolution	10,000 steps per syringe volume
Maximum dosing rate	16.6 mL/min
Software control	Kalliope™ software version 1.8 or higher