The CAMAG Derivatizer sets a new standard of reproducibility and convenience in reagent transfer onto TLC/HPTLC plates by employing a unique “micro droplet” spraying technology (patent pending). The device is suitable for all common reagents. In addition to the unsurpassed homogeneous reagent distribution, the Derivatizer offers other advantages as compared to manual spraying and immersion.
• Environmentally friendly and safe handling through a closed system
• Reproducible and user-independent results
• Low reagent consumption (2–4 mL) through efficient operation
• Intuitive handling and easy cleaning
How it works
At the push of a button, the transparent hood automatically lifts so that the user can remove the plate tray and place the TLC/HPTLC plate on it. After the tray is inserted into its original operating position, the hood is lowered and completely sealed to prevent aerosols/spraying reagent from leaking to the outside. The user fills the required reagent volume into the appropriate nozzle, selects the optimum spraying model and starts the reagent transfer. To meet the divergent physicochemical properties of the reagents, e.g. viscosity, four different color-coded nozzles are available, and the user can select from six spraying modes. The nozzle generates an extremely fine reagent aerosol, which evenly distributes in the chamber and gradually settles down on the TLC plate.
Any residue remaining in the gas phase is automatically aspirated by the integrated pump and collected in the wash bottle behind the instrument.
After the reagent transfer has been completed, the hood lifts at the push of a button and the TLC/HPTLC plate can be removed for further processing.
Derivatization means another step in the process of planar chromatography, consequently causing an increase in variance. Even with a trained, expedient technician manually spraying a reagent, the relative standard deviation (RSD) of the measured values rises up to 12%. Using the CAMAG Derivatizer, the RSD increases by less than 5%, thus providing very reproducible results, equal to those obtained with the Chromatogram Immersion Device, yet consuming considerably less reagent.
The following most common reagents have been tested and validated by the CAMAG laboratory for use with the CAMAG Derivatizer:
• Sulfuric acid reagent (10 % in methanol)
• Anisaldehyde reagent
• Natural product reagent
• Polyethylene glycol solution
• Iodine solution (0.5% in ethanol)
• Dragendorff reagent
• Fast blue salt B reagent
• Ehrlich’s reagent
• Phosphomolybdic acid reagent
• Ninhydrin reagent
• Copper (II) sulfate reagent
• Aniline-diphenylamine-phosphoric acid reagent
• Vanillin reagent
• Potassium hydroxide solution (5% in methanol)
• Aqueous solutions (enzymatic solutions, etc.)
For alternative spraying reagents and reagents that are problematic to spray, please download our recommendation list.
The CAMAG Derivatizer is available for two different plate formats (20 x 20 and 20 x 10 cm).
To get information about: «CAMAG Derivatizer» contact us by phone (044) 229 15 31 or fill in the form.