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Sample application

Description
Application of samples, reference standards and/or reference materials is the first step in the workflow of TLC/HPTLC and it affects significantly the quality of the result at the end of the process.

The choice of the application technique and the device depend on the requirements of precision, sample volumes, number of analyses and the desired grade of automation.

Spotwise sample application using a fixed volume capillary is the simplest way. Sample volumes of 0.5 to 5 μL can be applied as spots onto conventional layers without intermediate drying, on HPTLC layers it is up to 1 μL per spot. It is recommended to guide the capillary by means of the Nanomat 4.

Bandwise application by spray-on techniques allows the application of significantly larger volumes. Starting zones in the form of narrow bands ensure the best resolution that can be achieved with the chromatographic system selected.

Very large sample volumes or samples with a high matrix content can be sprayed-on in the form of rectangles with the Automatic TLC Sampler (ATS 4) which, prior to chromatography, are focused into narrow bands by a short development step with a solvent of high elution strength.
Product list
CAMAG Nanomat 4 and Capillary Dispenser

CAMAG Nanomat 4 and Capillary Dispenser

The Nanomat 4 serves for easy application of samples in the form of spots onto TLC and HPTLC layers, precisely positioned and without damage to the layer. The actual sample dosage is performed with a disposable capillary pipette
LINOMAT 5

LINOMAT 5

With the Linomat 5 samples are sprayed onto TLC/HPTLC plates in the form of bands with nitrogen or compressed air. Sample application is automatic, only changing the syringe is manual.
Automatic TLC Sampler 4 (ATS 4)

Automatic TLC Sampler 4 (ATS 4)

The requirements for an instrument serving this purpose, i.e. precision, robustness during routine use and convenient handling are fully met by the Automatic TLC Sampler 4.
Downloads
Sample Application.pdf