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Derivatization

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It is an inherent advantage of TLC/HPTLC that all fractions remain stored on the plate and can be readily derivatized after chromatography. Substances that do not respond to visible or UV light can be rendered detectable. In many cases, substances or classes of substances can be identified by specific reagents, enabling their selective detection.
Pre-chromatographic derivatization is possible by overspraying the sample application zones with the Linomat 5 or the Automatic TLC Sampler ATS 4. For the transfer of liquid reagents for postchromatographic derivatization, one can choose between spraying or dipping. Dipping and automated spraying are the preferred techniques, particularly when a quantitative evaluation is intended. Usually reagent transfer by spraying can not be circumvented when two reagent solutions have to be applied in sequence without intermediate drying, for instance diazotation followed by coupling.

Whenever reagents are transferred by spraying, an efficient reagent mist removing device should be used to protect laboratory personnel against poisonous or irritating sprays or solvent vapors.

In most cases the derivatization reaction needs to be completed by heat treatment. Heating the plate at the desired temperature with a plate heater specifically designed for this purpose is highly recommended. An oven used for this purpose will become permanently contaminated.

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