CHM® BIO-tr@ns blotting membrane
Blotting membranes are used widely in molecular biology, biotechnology and genetics, as a method of transferring proteins, DNA or RNA. CHMLAB offers 3 kinds of BIO-tr@ns blotting membranes PVDF transfer membrane, nitrocellulose membrane and nylon membrane. Selecting the appropriate membrane is critical to the success of a nucleic acid or protein transfer procedure. The several types of Bio-tr@ns transfer membranes exhibit different performance characteristics which can directly affect the outcome of a specific technique.
We can custom blotting membrane, like the shape, size, contents, package and different use as per your requirements.
CHM® BIO-tr@ns NC
CHM® BIO-tr@ns PVDF
CHM® BIO-tr@ns NY
BIO-tr@ns Pure NC membrane
CHMLAB Bio-tr@ns Pure NC membrane is one of the most widely used in analytical and research applications. Minimize amounts of wetting agent and have a lower water extractable content. The high sensitivity of pure cellulose nitrate ensures excellent results in all the transfers, specially in protein blotting.
Features:
· 100% Pure Nitrocellulose
· For process requiring optimum resolution
· Compatible with Chromogenic, Radioactive, Fluorescent detection methods
· Excellent strength
· No detergents added
· Binding Interaction: hydrophobic & electrostatic
Applications:
· Westerns
· Protein & inmunoblotting
· Northerns
· Southerns
· Dot/slot blots
CHMLAB Bio-tr@ns Supported NC membrane recommended for DNA/RNA/Protein transfers with procedures requiring rigorous handling.
Features:
· Supported Nitrocellulose
· Multiple reprobings
· Nucleic acid binding up to 100 µg/cm2
· High sensitivities, low backgrounds
Applications:
· Multiple re-hybridations
· Colony/plaque lifts
· Northerns
· Southerns
· Dot/slot blots
· Biotinylated detection systems
· Chemiluminescent detection systems
BIO-tr@ns PVDF membrane
CHMLAB Bio-tr@ns PVDF membrane has high protein adsorption, so proteins during transfer or reprobing won’t be lost. Open pore structure makes accessing bound proteins and removing unbound probes easily. Membranes optimized for fluorescent blots dramatically increase signal for high sensitivity in quantitative, multiplexing applications.
Features:
· Composition unsuported Polyvinylidene Fluoride
· Ideal for protein sequencing
· Chemical resistance
· No discoloration
· Nonflammable
· Hydrophobic
Applications:
· Western blots
· Western blotting
· Binding assays
· Amino acid analysis
· N-terminal protein sequencing
· Dot/slot blotting
· Glycoprotein visualization
· Lipopolysaccharide analysis
BIO-tr@ns NYLON membrane
CHMLAB Bio-tr@ns Neutral Nylon.
Open pore structure permits maximum accessibility of target sequences to the probe and allows efficient removal of unhybridized probe, thereby reducing background.
Features:
· High strength
· High sensitivity
· Versatile adsorption properties
· Chemical resistance
· Hydrophilic
Applications:
· Colony/plaque lifts
· Dot/slot blotting
· Cell culture
· Clarification of aqueous solutions
· Chromogenic, radioactive, fluorescent detection systems
· Northerns
· Southerns
· Protein binding
· Microarrays
· Macroarrays
CHMLAB Bio-tr@ns Reprobing Charged Nylon - is an inherently charged nylon membrane, specifically designed to allow for numerous reprobings.
Features:
· Positively Supported charged nylon membrane
· Nucleic acid binding is 450 µg/cm2
· Provide consistent results through 12 or more reprobings
Applications:
· Northerns
· Southerns
· Radiolabelled and non-radiolabelled detection systems
· Multiple reprobings
· UV crosslinking
· Alkaline blotting
| Order information |
| BIO-tr@ns Pure NC membrane |
| | PURE NITROCELLULOSE | SUPPORTED NITROCELLULOSE |
| PORE SIZE | 0,45 µm | 0,2 µm | 0,45 µm | 0,2 µm |
| CIRCLES (mm) (50/box) |
| 82 | BCN045082K | BCN020082K | BCNS045082K | BCNS020082K |
| 85 | BCN045085K | BCN020085K | BCNS045085K | BCNS020085K |
| 132 | BCN0450132K | BCN020220220V | BCNS0450132K | BCNS020132K |
| 137 | BCN0450137K | BCN020137K | BCNS0450137K | BCNS020137K |
| SHEETS (cm) (5/box) |
| 15 x 15 cm | BCN045150150V | BCN020150150V | BCNS045150150V | BCNS020220220V |
| 20 x 20 cm | BCN045200200V | BCN020200200V | BCNS045200200V | BCNS020200200V |
| 22 x 22 cm | BCN045220220V | BCN020220220V | BCNS045220220V | BCNS020220220V |
| ROLLS (1/PACK) |
| 30 cm x 3 m | BCN04530300R | BCN02030300R | BCNS04530300R | BCNS02030300R |
| 20 cm x 3 m | BCN04520300R | BCN02020300R | BCNS04520300R | BCNS02020300R |
| BIO-tr@ns PVDF membrane |
| PORE SIZE | 0,45 µm | 0,2 µm |
| SHEETS (cm) (5/box) |
| 15 x 15 cm | BPV045150150V | BPV020150150V |
| 20 x 20 cm | BPV045200200V | BPV020200200V |
| 22 x 22 cm | BPV045220220V | BPV020220220V |
| ROLLS (1/PACK) |
| 30 cm x 3 m | BPV04530300R | BPV02030300R |
| 20 cm x 3 m | BPV04520300R | BPV02020300R |
| BIO-tr@ns NYLON membrane |
| | NYLON | NYLON REPROBING CHARGED |
| PORE SIZE | 0,45 µm | 0,45 µm |
| CIRCLES (mm) (50/box) |
| 82 | BNY045082K | BNYR045082K |
| 85 | BNY045085K | BNYR045085K |
| 132 | BNY0450132K | BNYR0450132K |
| 137 | BNY0450137K | BNYR0450137K |
| SHEETS (cm) (5/box) |
| 15 x 15 cm | BNY045150150V | BNYR045150150V |
| 20 x 20 cm | BNY045200200V | BNYR045200200V |
| 22 x 22 cm | BNY045220220V | BNYR045220220V |
| ROLLS (1/PACK) |
| 30 cm x 3 m | BNY04530300R | BNYR04530300R |
| 20 cm x 3 m | BNY04520300R | BNYR04520300R |
| | | | |
To get information about: «Blotting membranes» contact us by phone (044) 229 15 31 or fill in the form.