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TLC Scanner 4

Full description

The CAMAG TLC Scanner 4 is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects.


Key features
• Measurement of reflection, either in absorbance or fluorescence mode
• Object formats up to 20 x 20 cm
• Spectral range from 190 to 900 nm
• Automatic start of all lamps: deuterium, halogen-tungsten, and high pressure mercury lamp
• Data step resolution 25–200 μm
• Scanning speed 1–100 mm/s
• Spectrum recording up to 100 nm/s
• Automatic selection of electronic amplification
• Rapid data transfer
• Meets all requirements to be used in a cGMP/cGLP environment
• IQ/OQ qualification and 21 CFR Part 11 ready

Evaluation with visionCATS
visionCATS controls the CAMAG TLC Scanner 4 and enables quantitative evaluation of the generated densitometric data. To determine the substance concentration in a sample, five different quantification functions (e.g. linear and polynomial) are available. Several scanning steps (e.g. scanning the plate after development and scanning the same plate after derivatization) and up to five different evaluations can be performed (with data obtained from single wavelength, multiple wavelengths or a combination of measurements in absorption and fluorescence detection mode).

The Scanner Ultimate Package
• Multi Wavelength: this feature offers the possibility to perform a multi-wavelength scan with up to 31 selected wavelengths or a combination of measurements in absorption and fluorescence detection mode.
• Scanner Quantification: this feature allows to quantify each individual substance on the plate. Five different quantification functions are available for evaluation to determine the concentration of the substance in a sample. In one analysis file up to five evaluation steps can be performed in multiple plate states (e.g. plate after development and same plate after derivatization).
• Spectrum Scanning: this feature includes the measurement of the spectrum of each individual substance on the plate including the evaluation of the substance purity by comparison with reference standard.

Examples with visionCATS

MWL-assign
Peak Assignment
The separated compounds are assigned. For quantification, data from the multi-wavelength scan at the optimum wavelength for each compound is used.



MWL-full-screen-3D


3D View and Peak Integration
Densitograms are displayed in 3D, top or front view. Several peak integration and baseline correction settings can be selected.


 grafik_optisches_system_scanner_4EN_text

Calibration Curve
For evaluation the best fitting calibration model is used. Quantitation can be done via peak height or area.

The Optical System

Any of the three light sources, high pressure mercury lamp, deuterium lamp, or halogen-tungsten lamp can be positioned in the light path by a motor drive. (1)

The signal of the measuring photomultiplier is continuously offset against the signal of the reference photomultiplier. (4 and 5) This compensates for lamp aging and short-time fluctuations. It also reduces the warm-up time required to reach lamp stabilization.

All components of the optical system, lamps, monochromator, scanning stage, and photomultiplier are mounted on one sturdy metal support. This ensures high precision of the detector signal.

For scanning at wavelengths below 200 nm it is advisable to flush the monochromator with nitrogen. The scanner is equipped to do this.

A monochromator bandwidth of 5 nm or 20 nm can be selected. 5 nm bandwidth is used for spectra recording, multi-wavelength scanning, and when spectral selectivity is required. (2) 20 nm bandwidth offers higher light intensity (improves the signal to noise ratio and thus the reproducibility of the measurement) and enables measurement of several fractions with slightly different absorption maxima in one scan.

The lens system with 190 – 900 nm transmission range features automatic positioning for micro and macro slit sizes. This ensures that the light energy available with small slits in the micro position is almost the same as that for the corresponding slit in the macro position, which is four times larger. (3)

The light beam strikes the object at right angle. The photomultiplier is aligned at an angle of 30°. (5)
Product specification
Model TLC Scanner 4
Light sources Halogen-tungsten lamp, usable continuum 350 – 900 nm
  Deuterium lamp, usable continuum 190 – 450 nm
  High-pressure mercury lamp, spectral lines (248, 254, 265, 
  280, 297, 302, 313, 366, 405, 436, 546, 577, 579 nm)
  The lamp, which is positioned in the light path, is automatically 
  ignited. All lamps are current stabilized.
Pilot lamp and  The slit is automatically illuminated with visible light when the 
compartment  compartment illumination is switched on. The scanning 
illumination compartment is illuminated with a 4 W  tube emitting UV 254 nm 
  which the user can replace by a UV 366 nm or a white light tube.
Monochromator Concave holographic grating, 1200 lines/mm, bandwidth 
  selectable 5 or 20 nm, wavelength range 190 – 900 nm; 
  monochromator driven by step motor, reproducibility 
   of wavelength setting better than 0.2 nm, accuracy 
  better than 1 nm; connector for flushing with nitrogen.
  Maximum speed of spectra recording 100 nm/s.
Secondary filter Motor-driven filter wheel with three automatically selected 
  filters for the elimination of second order wavelengths; 400 nm 
  cut-off filter for fluorescence measurements; 
  three positions for user selected filters.
Scanning slit Revolving disk with 20 fixed apertures; length of slit images 
  selectable between 0.2 and 12 mm, width between 
  0.1 and 1.2 mm in 42 combinations.
Detector Two matched broadband photo multipliers, multi alkali type, 
  spectral sensitivity 185 – 900 nm.
Stage drive Independent in both directions by step motors, micro step 
  driven for smooth movement; reproducibility of positioning 
  better than 50 μm in Y-direction, better than 100 μm in 
  X-direction; maximum scanning speed 100 mm/s.
Mains voltage 115 V and 230 V selectable; 50/60 Hz; maximum energy 
  use 180 W (tungsten and mercury lamp ignited).
A/D converter 16 bit, 2-channel A/D converter, 100 ms per double conversion.
Connections/ RS-232 serial port for PC communication
interfaces  
Dimensions W x D x H: 590 x 650 x 367 mm
Weight 39 kg
Downloads
Chromatogram Evaluation.pdf
Brochure_TLC_Scanner_4_2011_EN.pdf

To get information about: «TLC Scanner 4» contact us by phone (044) 229 15 31 or fill in the form.
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