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Derivatization
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Derivatization
Description
It is an inherent advantage of TLC/HPTLC that all fractions remain stored on the plate and can be readily derivatized after chromatography. Substances that do not respond to visible or UV light can be rendered detectable. In many cases, substances or classes of substances can be identified by specific reagents, enabling their selective detection.
Pre-chromatographic derivatization is possible by overspraying the sample application zones with the Linomat 5 or the Automatic TLC Sampler ATS 4. For the transfer of liquid reagents for postchromatographic derivatization, one can choose between spraying or dipping. Dipping and automated spraying are the preferred techniques, particularly when a quantitative evaluation is intended. Usually reagent transfer by spraying can not be circumvented when two reagent solutions have to be applied in sequence without intermediate drying, for instance diazotation followed by coupling.
Whenever reagents are transferred by spraying, an efficient reagent mist removing device should be used to protect laboratory personnel against poisonous or irritating sprays or solvent vapors.
In most cases the derivatization reaction needs to be completed by heat treatment. Heating the plate at the desired temperature with a plate heater specifically designed for this purpose is highly recommended. An oven used for this purpose will become permanently contaminated.
Product list
CAMAG Derivatizer
The CAMAG Derivatizer sets a new standard of reproducibility and convenience in reagent transfer onto TLC/HPTLC plates by employing a unique “micro droplet” spraying technology (patent pending)
Sprayers and TLC Spray Cabinet 2
The CAMAG TLC Spray Cabinet 2 ensures the complete removal of reagent mist while spraying TLC plates.
Chromatogram immersion device 3
The chromatogram must be immersed and withdrawn at a controlled uniform speed. By maintaining a well defined vertical speed and immersion time, derivatization conditions can be standardized
TLC PLATE HEATER 3
The CAMAG TLC Plate Heater 3 is designed for heating TLC/HPTLC plates to a selected temperature after a derivatization reagent has been applied
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Derivatization.pdf
Applications
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ВЭТСХ – инструментальная ТСХ (Презентация)