Chromatogram development, CAMAG

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Chromatogram development

Description

Elution in reproducible standardized conditions is a key condition for obtaining high-quality results.

Selection of the «proper» chamber is done during method development and generally follows «practical» considerations such as which chamber is available, which one must be used due to an SOP or following a guideline, or which one has been used in the past if a results comparison is to be made. However, a focus should also be on economical aspects such as time requirement and solvent consumption and on reproducible conditions.

The Horizontal Developing Chambers have proven to be exceptionally economical, flexible and reproducible in operation. Although designed for applications where the plate is developed from two sides, they are also suitable for single-sided developments in unsaturated, saturated and sandwich configuration as well as for preconditioning of HPTLC plates. Please note that results are not comparable to vertical developing chambers.

The Automatic Developing Chamber 2 (ADC 2) is a software-controlled device for reproducible plate development. This instrument does not only eliminate any effects of the operator when introducing the plate into a saturated chamber, but also the activity of the layer prior to start of chromatography can be set and drying of the developed plate is rapid, homogeneous, and complete. For development a conventional 20 x 10 cm Twin Trough Chamber is used. This way chamber geometry and chromatographic conditions of already existing analytical procedures can be retained, but environmental and operational effects are standardized.

In case the sample contains polar and non-polar components, which must be separated in the same analysis, the principle of Automated Multiple Development (AMD 2) can be employed. Development is performed on the basis of a solvent gradient from polar to non-polar over several steps with intermediate drying.

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