Sample application, Біотехнологія та біохімія

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Sample application

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Application of samples, reference standards and/or reference materials is the first step in the workflow of TLC/HPTLC and it affects significantly the quality of the result at the end of the process.

The choice of the application technique and the device depend on the requirements of precision, sample volumes, number of analyses and the desired grade of automation.

Spotwise sample application using a fixed volume capillary is the simplest way. Sample volumes of 0.5 to 5 μL can be applied as spots onto conventional layers without intermediate drying, on HPTLC layers it is up to 1 μL per spot. It is recommended to guide the capillary by means of the Nanomat 4.

Bandwise application by spray-on techniques allows the application of significantly larger volumes. Starting zones in the form of narrow bands ensure the best resolution that can be achieved with the chromatographic system selected.

Very large sample volumes or samples with a high matrix content can be sprayed-on in the form of rectangles with the Automatic TLC Sampler (ATS 4) which, prior to chromatography, are focused into narrow bands by a short development step with a solvent of high elution strength.

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